Wednesday, October 30, 2019

Science fiction and films Essay Example | Topics and Well Written Essays - 3000 words

Science fiction and films - Essay Example The cyber punk nature of the film ‘Blade Runner’ is explicable as it involves the speculation of role of computerised human clones of the future, which is stimulating. As the film is associated with rave/techno subculture and substitutes enthusiastic blathering about technology, the cyber punk nature of the film was expressed in the way of narration and visuals. As the film involves the exciting about right things and a proper and respectful way of hacking the talent, the film excels in being a science fiction genre that explicitly talks about computerised clones of humans. As the computerised clones in the film are expressed in a highly criminalised manner, the necessity of expression of the criminal nature needs some symbolic lighting and expression that involves the value of life and humanity. As the lead character in the film Deckard prowls in the steel and micro chip jungle of 21st Century, the cyber punk nature of the film is explicitly seen in every inch of the na rration and story. However, the expression of humanity, detachment and precious nature of life involved inherently in the film give it a combined colour of film noir sub genre in addition to cyber punk sub genre for a science fiction genre film.... In addition to that the theme of the film concentrates on the replicant nature of Deckard and the reaction of Gaff about Deckard's dreams. As Gaff will be in control of a replicant the plot is to field an android to destroy unwanted androids. From here, the film introduces film noir sub genre in addition to the cyber punk sub genre in a science fiction genre film. The film noir genre and content of the film lies in the criminal background of replicants and a state killer tracking down them. As the plot is to murder the artificial people for their illegal acts (wanting to live more than the stipulated time against the wishes of the administration), the film noir nature of the film is expressed well by frequent voice over in the film. 2 In addition to that the general brutality (the brutality of four replicants, whom Deckard has to execute) violence and harsh dialogue are present in the characters. The harsh dialogue can be seen in the expression of the leader of the replicants who confront with their creator. As the inherent activities of replicants are related to crime and horror, the lead character also express some attributes of film noir with a trench coat and a gun. In addition to that there are anti hero characters in Deckard as he drinks too much, loner and does not trusts authorities. Though Deckard is a detective, the harsh dialogues like "They do not advertise for killers. I am not in the business. I am the business. If You are not a cop, you are little people", makes the narration of the film to have a noir type of content. One more aspect that establishes the noir nature in the film is that the violence is brutal and graphic. As the duty of Deckard is fatal (he is to kill the

Monday, October 28, 2019

Love, Death and Fascist Iconography Essay Example for Free

Love, Death and Fascist Iconography Essay To the fascist artist, his or her own art is merely a vessel that encapsulates his or her own socially-motivated beliefs. The works of Yukio Mishima and Leni Riefenstahl, for instance, embody the essence of their individual nationalities at the height of their individual careers—nationalities that might transcend origin and geography, yet extol   culture, tradition, ritual and society.   Mishima and Riefenstahl exhibit incredible parallelism in their works. Mishima, in his short story Patriotism, describe the human form with such detail and meticulousness reminiscent of Riefenstahl’s style in her 1930’s films. Much like Riefenstahl’s The Last of the Nuba, the naked body is depicted as a means of fascist iconography—the body, perfect and pure in its own way, merges with a bigger community of bodies of like characteristics (Evans 31). Susan Sontag’s treatise on the works of Riefenstahl, Fascinating Fascism, breaks down the latter’s appreciation and fascination of the strong and beautiful Nuba figure as examples of Nazi ideology corresponding to the idea of aesthetics for fascists. Sontag writes, â€Å"Riefenstahl’s portrait of them evokes some of the larger themes of Nazi ideology: the contrast between the clean and the impure, the incorruptible and the defiled, the physical and the mental, the joyful and the critical (par. 27).† This description is echoed in Mishima’s Patriotism, when the lead character Takeyama decides to commit seppuku—ritual suicide by disembowelment—as an act of redemption from the dishonor incurred when his comrades formed alliances with the mutineers.   Mishima’s imagery of Takeyama and his wife Reiko’s preparations for the ritual, as well as the metaphors he uses in describing the couple’s physical forms conveys a fascination for perfection and beauty a la Riefenstahl—remarkable symbols of fascist iconography, as Sontag would consider. In its own effect, Takeyama, Reiko and the Nuba become examples of the â€Å"master race†, where everyone is beautiful, strong, and does not age (Trimborn McCown 256). Mishima and Riefenstahl’s works are also parallel in such that their depictions of death are detailed, beautiful and almost enthralling.     In Patriotism, death in is portrayed as a reward, a happy and celebrated ceremony that requires the utmost preparation. In the Nuba culture, death is a central event, along with fighting. In the Japanese culture, as evidenced by Mishima’s paramount detail in describing Takeyama’s death, death is a way for one to demonstrate his or her enduring loyalty and nationalism to the Imperial system. Death in both Mishima and Riefenstahl’s works surpass life’s merriest events—birth, marriage, love—at times besting even life itself. Mishima writes, â€Å"All around, vastly and untidily, stretched the country for which he grieved. He was to give his life for it. But would that great country, which he was prepared to remonstrate to the extent of destroying himself take the slightest heed of his death? He did not know; and not matter (par. 63).† Takeyama’s examination of self not only mirrors the authors suggestions of his own death wish, it also echoes the sentiments of those under the spell of a fascist ideology—their so-called patriotism—that converts their idea of nationhood as something not only territorial, but equally and incredibly spiritual. The young lieutenant and his bride chose their own death by seppuku, which may be seen as either an honorable and extremely devout approach, or as a self-destructive and deadly consequence of their fanaticism. Mishima unintentionally diverts the reader’s attention from this concept with his alluring illustrations of fascist ideals and concepts, again reminiscent of Riefenstahl’s imagery in her Nazi films. Sontag would consider it an absolute expression of fascist art, in the sense that it â€Å"glorifies surrender, exalts mindlessness, and glamorizes death (par. 36).† Above everything, Mishima verifies in his work and own life the burden that comes with the duty to abide by cultural tradition—duty can be all in one beautiful or ugly, life or death. Works Cited Evans, Mark. Movement Training for the Modern Actor. London, UK: Taylor and Francis,   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   2008. Mishima, Yushio. â€Å"Patriotism.† Trans. Geoffrey W. Sargent. Mutantfrog Travelogue. 27   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   June 2009. http://www.mutantfrog.com/patriotism-by-yukio-mishima/ Sontag, Susan. â€Å"Fascinating Fascism. â€Å" University of California Santa Barbara. 27 June 2009.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   http://www.history.ucsb.edu/faculty/marcuse/classes/33d/33dTexts/SontagFascinFas  Ã‚  Ã‚   cism75.htm Trimborn, Jurgen McCown, Edna. Leni Riefenstahl: A Life. New York: MacMillan, 2007.

Saturday, October 26, 2019

heroin Essay -- essays research papers

Society today has been clouded and somewhat overtaken by social drugs. Wherever you may look, a drug is being used, whether it is more commonly a cigarette being smoked on the street, or the covert teens smoking marijuana in secluded areas. In any case, there is not one person who can say drug use is not prevalent, since society has made it clear through news, music and everyday life. However, there are certain drugs that seem to be worse than others, and society once again has taught us that through our laws and restrictions. The worse the drug, the more you pay for having it. Basically, drugs have become a part of our life, and you never know when they can land on your doorstep.   Ã‚  Ã‚  Ã‚  Ã‚  An illegal drug, which has taken firm ground in society, is heroin. Heroin is one of the most used â€Å"hard† drugs in society today. Even though it is illegal, surveys have proved that many people have done and are still doing heroin. The worst part is that heroin is classified as having the worst addiction and withdrawal symptoms than any other drug. Heroin is known as â€Å"H† or â€Å"Brown†, and is related to other drugs because of its elementary foundation.   Ã‚  Ã‚  Ã‚  Ã‚  Heroin is related to morphine and codeine, since all three are derived from the opium poppy plant. They are called opiates, and are found in the dried â€Å"milk† of opium poppy seeds. Morphine and codeine are both very effective painkillers and are used for medical uses, such as cough medicines and after surgery painkillers. However, these drugs can form a quick dependency in the people that use them, and therefore must be used with caution.   Ã‚  Ã‚  Ã‚  Ã‚  The opium poppy plant and its use date back as far as 6000-year-old Sumerian texts. The Sumerians are an ancient people, and referred to the poppy plant as the â€Å"joy plant.† In either the 7th or 8th centuries, it is believed that Arab traders brought opium to China, where it was used as medicine. At about the 17th century, it was realized that opium could be smoked. Later on m the Portuguese, then the British were supplying China with most of its opium. Britain would also sell opium to India to be smuggled into China, and in turn, the Chinese government would destroy the opium imports before it reached China. This little plant sparked two wars between China and Britain, and in conclusion, Britain received Hong Kon... ...tes to relieve withdrawal symptoms, and the person can be okay without the drug. Within detox, a person can talk to a counselor, so while their body is becoming physically better, psychologically they can be helped. Heroin addicts definitely need psychological help. Their minds tend to focus on getting more heroin and doing whatever they can to get it. This is so even if it means stealing or hurting friends and family. In conclusion, heroin is a disgusting drug, which can harm you and take away important time of your life. It is easy to overdose, you mess with the way you think, and the way your nervous system works. Heroin is the most addictive drug out there because the symptoms you get when you don’t have it are absolutely horrible. Synthetic opiates such as LAAM and methadone are drugs that can help a person get the physical experience, but still becoming free of the drug itself. The abuse of Heroin can destroy a person’s life, their friendships, and their bodies. When someone is addicted to heroin, they not only hurt themselves, they hurt the people who love them. Heroin Abuse and Addiction.† www.NIDA.com  Ã‚  Ã‚  Ã‚  Ã‚  Ã¢â‚¬Å"Opiates/Heroin.† www.druginformation.com heroin Essay -- essays research papers Society today has been clouded and somewhat overtaken by social drugs. Wherever you may look, a drug is being used, whether it is more commonly a cigarette being smoked on the street, or the covert teens smoking marijuana in secluded areas. In any case, there is not one person who can say drug use is not prevalent, since society has made it clear through news, music and everyday life. However, there are certain drugs that seem to be worse than others, and society once again has taught us that through our laws and restrictions. The worse the drug, the more you pay for having it. Basically, drugs have become a part of our life, and you never know when they can land on your doorstep.   Ã‚  Ã‚  Ã‚  Ã‚  An illegal drug, which has taken firm ground in society, is heroin. Heroin is one of the most used â€Å"hard† drugs in society today. Even though it is illegal, surveys have proved that many people have done and are still doing heroin. The worst part is that heroin is classified as having the worst addiction and withdrawal symptoms than any other drug. Heroin is known as â€Å"H† or â€Å"Brown†, and is related to other drugs because of its elementary foundation.   Ã‚  Ã‚  Ã‚  Ã‚  Heroin is related to morphine and codeine, since all three are derived from the opium poppy plant. They are called opiates, and are found in the dried â€Å"milk† of opium poppy seeds. Morphine and codeine are both very effective painkillers and are used for medical uses, such as cough medicines and after surgery painkillers. However, these drugs can form a quick dependency in the people that use them, and therefore must be used with caution.   Ã‚  Ã‚  Ã‚  Ã‚  The opium poppy plant and its use date back as far as 6000-year-old Sumerian texts. The Sumerians are an ancient people, and referred to the poppy plant as the â€Å"joy plant.† In either the 7th or 8th centuries, it is believed that Arab traders brought opium to China, where it was used as medicine. At about the 17th century, it was realized that opium could be smoked. Later on m the Portuguese, then the British were supplying China with most of its opium. Britain would also sell opium to India to be smuggled into China, and in turn, the Chinese government would destroy the opium imports before it reached China. This little plant sparked two wars between China and Britain, and in conclusion, Britain received Hong Kon... ...tes to relieve withdrawal symptoms, and the person can be okay without the drug. Within detox, a person can talk to a counselor, so while their body is becoming physically better, psychologically they can be helped. Heroin addicts definitely need psychological help. Their minds tend to focus on getting more heroin and doing whatever they can to get it. This is so even if it means stealing or hurting friends and family. In conclusion, heroin is a disgusting drug, which can harm you and take away important time of your life. It is easy to overdose, you mess with the way you think, and the way your nervous system works. Heroin is the most addictive drug out there because the symptoms you get when you don’t have it are absolutely horrible. Synthetic opiates such as LAAM and methadone are drugs that can help a person get the physical experience, but still becoming free of the drug itself. The abuse of Heroin can destroy a person’s life, their friendships, and their bodies. When someone is addicted to heroin, they not only hurt themselves, they hurt the people who love them. Heroin Abuse and Addiction.† www.NIDA.com  Ã‚  Ã‚  Ã‚  Ã‚  Ã¢â‚¬Å"Opiates/Heroin.† www.druginformation.com

Thursday, October 24, 2019

What achieving a degree means to me :: essays research papers

â€Å"What achieving a degree means to me† Hello my name is ______________ I am a twenty nine year old married mother of two. I’ve been married for twelve years and I have an eight years old boy and a two years old girl. Since I was nineteen years old in the fall of 1991 I’ve attempted to earn a college degree attending first Tarrant County Junior College and second Weatherford College stop and starting back again several times over the years trying to reach my goal. In the summer of 1995 when my oldest was two my husband and I moved our mobile home on to new land, which required my assistance helping out financially. I started working for a great company that I loved, Auto Rail Services of Texas they are an afflation of Burlington Northern Santa Fe Railway were I worked part time in the evenings doing data entry. Six months later I started working full time as the assistant office manager in charge of the day-to-day operations. I was sent to Tarrant County Junior College to gain a certificate in Microsoft Offic e applications. I continued to advance with in the company and enjoyed working there but in the July of 1998 the company suffered financially when Ford Motor Company moved there business to Union Pacific Railway, which caused downsizing in the company I was laid off. I contemplated going back to school after this but couldn’t financially afford to stop working full time. The following October I became pregnant with our second child and after her birth my husband and I decided to downsize our financial situation so that I could remain home with the baby. When the child was seven months old I took a part time position in my aunts security company where I assisted in the office and was able to bring my child with me. Not long after my aunts company was forced to go out of business, which caused me to relay on unemployment compensation, threw Texas Workforce Commission there I was informed I could return to college and receive assistance with childcare. I began all the necessary proceedings for the program and was informed before I could actually get the assistance I would need to pass the TASP test a mandatory test required before you are eligible to receive a degree in the State of Texas. Having passed all but the math section of the test I decided I would take a preparatory tasp math class.

Wednesday, October 23, 2019

Analysis of Sonnet 75 (Amoretti) by Edmund Spenser Essay

Sonnet 75 is taken from Edmund Spenser’s poem Amoretti which was published in 1595. The poem has been fragmented into 89 short sonnets that combined make up the whole of the poem. The name Amoretti itself means â€Å"little notes† or â€Å"little cupids. † This poem is said to have been written on Spenser’s love affair and eventual marriage to Elizabeth Boyle, his second wife. Sonnet 75 centers on the immortality of spiritual love and the temporality of physical love. In the seventy-fifth sonnet of Amoretti, the line scheme of three quatrains and a rhyming couplet is employed. The rhyme scheme is the typical Spenserian sonnet format of the first quatrain being ABAB, the second being BCBC, the third CDCD and the couplet EE. It follows the meter of the iambic pentameter. The first quatrain has a narrative feel to it because of the beginning â€Å"One day I †¦Ã¢â‚¬  The second quatrain starts with a dialogue by a female, most probably the beloved. The third quatrain is an answer again in a conversation because of the use of â€Å"quoth I. † The couplet at the end gives the conclusion like a fact because it uses the present participle tense. Sonnet 75 is a lyric because it tells of the poet’s personal experience. Spenser writes this sonnet in the typical Petrarchan style. It is written in the pursuit of a woman whom he loves. The rhyme scheme coincides with the Petrarchan model. The whole sonnet reeks of the use of imagery. The very opening lines, â€Å"One day I wrote her name upon the strand, But came the waves and washed it away:† create a vivid image of the sea-side. Other excerpts from the sonnet that produce a visual effect are â€Å"decay,† â€Å"wiped out,† â€Å"die in dust,† and â€Å"in the heavens write your glorious name. † while the first three evoke negative images, the last one paints a beautiful, fantastical picture in the mind. The short sonnet incorporates alliteration very often, for instance, â€Å"die in dust,† â€Å"verse your virtue,† â€Å"love shall live,† â€Å"later life,† etc. The sounds that have been continuously been employed are /d/, /w/ and /l/. Alliteration throughout is helping form the images. The /w/ sound in â€Å"waves and washed it away† help create an audio impact of the swishing waves. The repetitive /d/ gives an ominous sound and is used for all such words, for example â€Å"decay,† â€Å"die,† â€Å"death,† etc. There is also repetition of certain words. â€Å"Vain† appears twice in one line. Then, the concept of mortality is conveyed through â€Å"mortal,† â€Å"immortalize† and â€Å"eternize. † The sonnet is rife with symbolism. The sea alludes to the distance that is between the lover and his beloved which is causing pain to the lover. The writing on the sand refers to the lover’s insistence on making a worldly impact on his beloved. The waves are a constant reminder of the cruelty of love, haunting again and again. By washing away the name of the beloved, the waves act as torrents of torture. The sea-side or beach also symbolizes a peaceful, comfortable place where the lover unreservedly expresses himself. The lover’s writing on the sand can be a reference to man’s inherent desire to eternalize his being to be remembered forever. The waves here signify time. The erasing of the name by water signifies the transient nature of human life. It points towards the futility of man’s aspirations for immortality, especially poets who wish to be eternalized through their works. However, irrespective of how many times he may try to make his life meaningful, it is pointless. Everything is transitory and will eventually be destroyed. Personification is an important element in the sonnet. The sea or waves are given human qualities. It â€Å"washed,† and â€Å"made my paynes his pray. † Washing and preying or inflicting pain upon someone are human qualities. The poet has dexterously presented a contrast between the earthly and the celestial ideas and things. While in the first half of the poem, time and nature destroy the poet’s writing and attempts to immortalize it; in the second half the poet immortalizes his eternal, spiritual love through his writings. One of the indirect implications of the typical fifteenth century women being docile and subservient can be found in the waves being given a masculine quality. Normally, nature is associated with the female entity because both are responsible for giving and sustaining life. Here, however, the author’s reason for giving a masculine identity to nature must be because of the malignant role it is playing. Edmund Spenser’s Sonnet 75 from Amorreti is not only an exquisite piece of Elizabethan times, it portrays the quintessential poetry of the time as well. His optimal employment of literary techniques of form, rhyme, imagery, personification and alliteration give the sonnet a wholesome structure and an pleasant quality. Theme : When he writes her name on the sand, her name is washed away by the waves. He tries again and again but his all attempts when the tide is in will be washed. The lover here emphasize that allegorically; The tide represents â€Å"the time† and The sand of seashore represents his â€Å"memories† The word â€Å"tide† refers to the word â€Å"time† also in means of written and â€Å"sand† also refers to his memories because memory is a reflection of the past and it has a particular shape in minds to indicate particular moments and events which we experienced. but this shape in time becomes uncertain as the time passed memory skips over some important or trivial details. So everthing can be forgetton, ,n memory there can be nothing everlasting JUST LIKE HER NAME ON THE SAND OF SEASHORE.. the poet has a great longing to immortalize her name. But he deduces that nature wants to explain that love is temporary. Because when he wrote her name the tide washes it away. Then the lady speaks that his effort is in vain he can not change the intrinsic nature of the mortality. She believes every mortal thing will be perished. She is offended by his attempt to immortalize her. But the lover believes when the love becomes immortal her name will be written in heaven. THE AMORETTI. Edmund Spenser – Sonnet 75 One day I wrote her name upon the strand, But came the waves and washed it away: Again I wrote it with a second hand, But came the tide, and made my pains his prey. Vain man, said she, that doest in vain assay A mortal thing so to immortalize, For I myself shall like to this decay, And eek my name be wiped out likewise. Not so (quoth I), let baser things devise To die in dust, but you shall live by fame: My verse your virtues rare shall eternize, And in the heavens write your glorious name. Where whenas Death shall all the world subdue, Out love shall live, and later life renew. â€Å" One day I wrote her name upon the strand ’’ This a case of Hyperbaton because the normal order of wards has been changed . The ordinary syntactic order world have been â€Å"I wrote her name upon the strand’’. â€Å"Again I wrote it with a second hand, But came the tyde , and made my paynes his pray ’’ This is a case of Transferred Epithet [ Hypallage ] The epithet ‘ second ’ properly belongs to time rather than to hand . It may also a case of personification since ‘tide’ is seen as hunting down the efforts of the poet. ‘ For I myself shall like to this decay, And eek my name by urped out likeirse†. This is a case of simile . The poet points to the similarity between the destruction of the engraved name by the sea and the death caused to the lady by time, the similarity is explicitly stated by the use of word ‘like’. â€Å"Not so,’ quod I, â€Å" let baser things devize † To die in dust but you shall live by fame ’ – This is a case of antithesis where two opposed ideas are balanced against each other to the same effect . The two ideas contrasted are the idea of the meaner things being reduced to dust by time on the one hand, and the idea of the superior beloved ‘ transcending this by fame , on the other . â€Å"Where when as death shall all the world subdew, Our love shall live, and later life renew † It is a case of Antithesis since the two opposed ideas of the death of the world and the living of their love are balanced against each other. This is also a cause of Epigram . Ordinarily the idea of the renewal of love after life may seem absurd, but on thinking more deeply one realizes that their love would indeed be renewed by the later generations of lovers who would model their love on the poet’s.

Tuesday, October 22, 2019

To Be, or Not to Be Black Essay

To Be, or Not to Be Black Essay To Be, or Not to Be Black Essay To Be, or Not to Be Black â€Å"Are you black?† is a question that I have encountered in my life countless times. The following conversations are always so similar it is almost as if they were scripted. I answer â€Å"yes†, but the person proceeds to tell me why I am not black. They say that I am too light-skinned, that I have â€Å"white-people hair†, or that I do not act black. I always wonder what makes these people think that they have the authority to tell me who I am. Despite being annoyed by their ignorance, I explain how I am indeed a black person. Having to constantly deal with encounters such as these has conditioned me to be ready to prove that I am black to anyone that questions me. It is troubling that people have to provide reasons as to why they are black, but it seems as if that is how society is today. After my experiences and observations of the â€Å"black† world around me, it has become evident that the population of â€Å"true† black people is very limited. Being â€Å"black" involves meeting certain standards set by society, dealing with stereotypes on a daily basis, and the ever so constant struggle to climb the social ladder. According to the Merriam Webster Collegiate Dictionary, black means â€Å"of or relating to a race of people who have dark skin and who come originally from Africa† (Merriam-Webster). The Oxford English Dictionary defines black in a variety of ways ranging from â€Å"denoting a member of any dark-skinned group of peoples†, to â€Å"enveloped in darkness†, and â€Å"deeply stained with dirt; soiled, filthy, begrimed† (OED Online). If official dictionaries recognize that anyone from African descent is black, why is that society cannot? Discrepancies between the dictionary definition of black and society’s definition of black elicit the controversy of what makes someone black. It appears that society has unofficial requirements a person must meet in order to be considered black. A person must act a certain way, do certain things, and go through certain kinds of experiences a â€Å"true† black person would go through. A friend once told me that if I have not seen the movie Love and Basketball I am not black because every black person has seen it. That comment struck a nerve because it was ridiculous that according to her, the difference between being black and white was something as simple as seeing a movie. Another requirement black people have to meet is how a black person should look. Apparently, a black person should be dark skinned and should not have â€Å"good† hair. However, this is the complete opposite of reality because black people have evolved into many different looks and makes. Now it seems as if the number of â€Å"true† black people is slowly shrinking. Stereotypes play a role in the daily life of black people. Sadly, most of the time the negative stereotypes are what represent black people as a whole. As a result from the stereotyping, a lot of other cultures look down on black people and their ways. Some of the stereotypes claim that all black people are thugs, low-lives, basketball players, and all sag their pants. The striking fact is that most of the stereotypes exist in other cultures, yet the focus is always on black people. Negative stereotypes have such a prominent role in black culture that the positive outcomes in the black community are often dismissed. For example, some of the country’s best entertainers, athletes, and politicians, including the President, are black (Raspberry 315). Even if these â€Å"elite† black people have not gained respect from everyone, no one can deny the fact that they are successful. Many fall victim to the dismissal of success when they should be receiving praise for their accomplishments. It is almost as if all black people are being placed into a proverbial box of people that are bad influences. Stereotypes shou ld never be allowed to become a blanket term for a group of people. Everyone is unique and society needs to start acknowledging this fact. The Oxford English Dictionary’s

Monday, October 21, 2019

The Need to Care for the Environment Essays

The Need to Care for the Environment Essays The Need to Care for the Environment Paper The Need to Care for the Environment Paper Whether the issue is air pollution, endangered species, re-cycling, or clean water, all environmental scientists, educators, and policy-makers agree that the key to public understanding is public education. Without a thorough understanding of the problems facing the planet in terms of its fragile environment, humanity is on a collision course with disaster. It is not enough to warn people, impose economic sanctions, and establish strict regulations. People must understand why they should recycle, how they can take care of their waste, and what they can do to lessen their own individual impact on the earth. It is also essential that environmental education be appropriate and applicable to the person doing the learning. Too often, environmental education focuses on a topic far removed from the reality of the student. A child living in the mountains can have all the concern in the world for the manatee or the blue whale and be completely unaware of the forest degradation going on in her own mountain backyard. Likewise a Brentwood student can be vitally concerned about the nuclear waste dumps in the desert but still mindlessly damage her own unique environment. In order for environmental education to be truly effective it must be both relevant and applicable. Such awareness may be the only truly viable option that exists that can make a difference in saving or renewing the earths rapidly diminishing natural resources. Such education must be fully integrated so that the student can understand the interrelatedness of all aspects of the environment, including her role within it. Once a person understands that all life on earth, not just human life, is dependent on the health and protection of the environment, it is no longer possible to dismiss the planet as a nothing more than an object designed to meet human needs and desires. The most valuable time to begin such environmental education and awareness is with teenagers while they have not yet lost their sense of connection to all that surrounds them. In order to best communicate the importance of the environment and the need to care for it as vigilantly as human beings care for themselves, students must begin to learn at an early age to respect the earth, its environment and all of its inhabitants. The environment is not some abstract concept. That lesson must continue to be taught and learned throughout life in order to facilitate meaningful change. Each individual must understand the consequences of her actions on the surrounding environment, whether it is in a rural area or city. Teenagers are, admittedly, creatures of self-interest but certainly there can be no greater service to oneself than the assurance that there is clean air to breathe, fresh water to drink, healthy food to eat or sanitary housing. The foundation and encouragement of that self-interest is education. Beginning in the first years of school, children are taught the processes of life on this planet. Even very young kids can easily understand that a strong plant cannot grow in unhealthy soil or that trash does not simply disappear. There can be no more valuable lesson than how to not poison oneself through unthinking or greed-based actions. Active recycling is genuinely a better lesson than algebra and living more simply in the midst of a consumer-driven society is undeniably more useful than memorizing the dates of battles long since fought. Environmental education can and does make a difference and is a valuable ideal for the future.

Sunday, October 20, 2019

PAPER WRITING SERVICES

PAPER WRITING SERVICES Writing essays is a type of work that requires research, good writing skills, and proper essay structuring. All students are known to make common mistakes that can be easily avoided. Paper writing services on the other hand are able to ensure the top writing quality. We decided to enlist common mistakes students tend to make, so you can be aware of them and avoid them. The first and the most common mistake is failing in following instructions. The professors and teachers always provide clear instructions to what essay must be written about and following both the writing guidelines and deadlines are extremely important.   The second the most important mistake is failure to write the right kind of essay as every single essay type does serve completely different purposes. It is important to know the difference type between narrative and persuasive essays. Ignoring or choosing the wrong essay type is a failure. Third mistake is failing to take notes. This common mistakes leads to providing wrong type of essay both style and content. Fourth mistake is plagiarism issue as copying will not help you to get what you want meaning the good grades. Ordering essays from paper writing services will save you much time and effort, providing top quality paper. PAPER WRITING SERVICES When you get on line for a search of the paper writing service you can trust, you may get confused with the number of online writing service. There is no need to point out the importance of quality paper writing services that become really good help with your academic studies. We feel highly confident as our paper writing services have been on the web for quite a while. We receive paper writing requests all over the world and we are able to cover these requests with the help of our professional team of writers. It is also important to point out that our whole company is working towards your success. What does this mean? Being one of the best paper writing services, our company is determined to bring our clients the most positive results ever, and to make our customers 100% satisfied with the writing products and services they receive. Our professional team of writers consists of various professionals who have years of writing experience and are able to determine precisely what are the exact guidelines of the paper orders our clients give us. Another important part of our company’s job is delivering plagiarism free papers. For that reason, all completed papers go through automatic plagiarism checks. As you see doing all this massive work, we are completely worth that trust and our paper writing services are definitely worth trying.

Saturday, October 19, 2019

Logistics Software Application Essay Example | Topics and Well Written Essays - 1500 words

Logistics Software Application - Essay Example vey conducted by the International Air Transport Association (IATA) reveals that the global air cargo will increase by 4.8% annually as calculated from 2007 to 2011 including the Asia Pacific air cargo growth rate that is around 6.3%. However, the Boeing Company has reveals that in the year 2004 and 2005 the air cargo will grow around 6% annually up to 2024 globally. As mentioned by the HKIA, the industry related to the air cargo will develop about 13% annually and the cargo handled by the Hong Kong’s air cargo will increased up to 13% annually. The long-term research has reveal that the Civil Aviation Administration of China sates that the air traffic going toward Mainland will increase immensely and the cargo facility will be developed by 10% per annum. The total cargo will increased till 30 million tones till the year 2020 and also the Mainland will start exporting its goods through other direct flights. Thus the annual cargo transferred facility provided by the Hong Kong w ill not be used by the Mainland in future. â€Å"Hong Kong International Trade receives over 10,000 trucks and 15 vessels a day, about six million twenty-foot equivalent units (TEUs) a year† (Murty et al. 61-75). The logistics service providers include high demand related to their services in order to compete in this industry. They should have maintained high efficiency, high value added services, high quality of services and high security services. In order to provide these services, fully professional personnel must be hired who possess good hands on experience, knowledge as, well as super current information regarding facility providing. Also, the company has to provide manpower training to its employees, develop air freight information platforms, improve management mode, strengthening international collaboration, and continuous updating of standard to international level are all directions for developing Hong Kong airfreight logistics industry. E-commerce operation mode has affected the

Friday, October 18, 2019

Family Assessment Essay Example | Topics and Well Written Essays - 750 words

Family Assessment - Essay Example Caroline is 38 years old. She has a 15 year old daughter. Carol and her daughter lived alone until Carol’s recent marriage to Trey Reyes. Carol is a very successful Sales Manager and travels a lot with her company. Mrs. Janice Jackson and Carol have a very strong bond. Lenny is Mr. and Mrs. Jackson’s third child. Lenny Jackson is 33 years old married to Desiree and has one child, a son Marcus. Marcus is 9 years old. Shelldon Jackson is Mr. and Mrs. Jackson’s youngest child. Shelldon is 32 years old married to Leigha and together have a daughter 4 years of age. Lenny also has a son from a previous relationship. Lenny’s son Alex is 12 years of age. The Jackson family is extremely close. Mrs. Jackson is very involved in not only all of her children’s lives and activities but also those of her grandchildren. Mr. Jackson unfortunately is not allowed the same opportunity due to his rigorous work schedule. Mr. Jackson is a chef and has to be up early in th e morning to be at work for 4 am. So when he gets home he usually sleeps a lot of the time. Mr. Jackson is there whenever he can be. The Jackson Family is and always has been very female influenced and dominated. â€Å"A Woman’s intuition never steers wrong† is there family motto. The males have always taken the role as the main providers of the family. Mr. and Mrs. Jackson are very involved in their church community and scold their children when they are not. Mrs. Jackson was raised a strong Baptist believer. The children are not always able to be there because of work and busy schedules. Mrs. Jackson does not feel that is an appropriate excuse. There should always be time for God since He’s made time for you. The Jackson Family does manage to attend church together as a whole at least one Sunday out of the month. Developmental The Jackson Family has always declared home their safe haven. Which means no matter what mistakes you make in life, do not ever think h ome is no longer an option. Home in the Jackson Family is where the center and heart of love is. There is no problem too great to solve and no hurt too great that can’t be healed. Usually behind the solutions to every problem and every hurt was Mrs. Jackson. Mrs. Jackson is the backbone of the family, until about a year ago. About a year ago Mrs. Jackson was getting ready to leave work when all of sudden she could not catch her breath. After causing the serious concern of her co-workers an ambulance was called. Mrs. Jackson was taken to a nearby hospital and admitted. Mr. Jackson was notified and arrived right away. Not long after the entire Jackson Family was sitting in a waiting room waiting to hear news that would devastate them all. Mrs. Jackson kidneys were failing and she needed a kidney transplant right away. The entire family went through tests to see if they were matches none of them passed the test. Mrs. Jackson’s condition continued to get worse even on the dialysis treatment. Functional Mrs. Jackson has had to relearn how to live life. The change her family has seen in her is devastating. A once strong woman is now defeated and trying to survive. Now it’s the family’s turn to be the backbone. The only problem is Mrs. Jackson refuses to allow anyone to take her place even though the days become more and more difficult for her. Mr. Jackson finally decided to take a risky stand cutting his hours at work to be at home more with Mrs. Jackson to monitor her. This was of course against Mrs. Jackson’

History and the Current Debate on Fox Hunting Literature review

History and the Current Debate on Fox Hunting - Literature review Example The view that man is superior to animal was also pointed out by Aristotle who thought that only humans were â€Å"capable of rational deliberation† (Trevelyan 1922). Hunting is one of the characteristics that mankind holds ever since its existence. It can be traced back to the days of the nomads whose only means of survival was through hunting. But with time, the verb has gone through a drastic change from a means of survival to a sport and thus a necessity has been replaced by entertainment. The Homo erectus hunted for living whereas the man of today hunts because hunting is a hobby and a means of amusement. It is an out-door sport with the intention to exhibit one’s skills or a perfect answer to one’s boredom. But for Paget, hunting is not a sport played at leisure but a dangerous pursuit of an animal in the places where they actually exist freely and reproduce. He says that a shot is solely registered for a hunt and if a man actually deviates from this reason, he is not a hunter and not a part of the sport of hunting (Paget 1900). Hunting is divided into various groups and fox hunting has been placed above all the other forms and has been marked to be the best sports by a group of people. It was and still continues to be the favourite sport of the Britons. Fox hunting is a blood sport as the animal world calls it and is a subject of much controversy as it is the shooting of animals for the purpose of enjoyment (Gast 2006). In Britain, the history of fox hunting holds a large volume and can be traced back to the 17th century when it was at its highest peak. It was the greatest sport until 1914 which was the year when the World War One started and it marked the end of the golden age. It was now surrounded by controversies and a full fledge debate up rooted between the masses whether it should be made legal or not and thus it was a turning point in the history of Britain (Wallen 2006). The history of Fox hunting can be traced back to 1534, when farmers in Norfolk, England started hunting down foxes with the assistance of their hounds. They were trained to follow the fox’s scent and only a hound could kill it while the hunter watched because its meat was not considered edible. The fox hunters were not much attracted to the idea of killing the fox with their own hands. They rather watched the hound do the honors while they sat on their horses and enjoyed the view. The fox was not meant for eating but was a pest that threatened farmers’ livestock and crops (Gradiner 2006). Thomas Fownes was the first man with a pack specifically for fox hunting. He sold it to a breeder in Yorkshire who further spread the transaction and by the middle of the century, several packs were seen that were exclusively kept for chasing down the foxes (Paget 1900). During the eighteenth century, fox hunting had replaced stag hunting and had spread out till Pytchley under the Lordship of Althrop (Trevelyan 1922). It had become the fa vourite sport of the riches who took it as a means of showing their wealth and valour. It was the sport of the higher class that existed in the society. There was a gradual division of class and culture and the upper class now aimed to live a much lavish life. They now, owned colossal estates and houses and enjoyed hunting because they were rich enough to buy and maintain horses and hounds (Gast 2006). The year 1753, is considered to be mark as an important era of fox

Thursday, October 17, 2019

Water supply in the USA Research Paper Example | Topics and Well Written Essays - 2000 words

Water supply in the USA - Research Paper Example The observation is that climate change further worsens existing pressures on water supplies in all of the United States, relating to the way Americans routinely use water not just for consumption, but also for a host of economic activities, including agriculture, manufacturing, and the generation of power, among many uses. The general problem relates to the overall impact of climate change relating to elevating the demand for water in all areas of the United States, while at the same time exerting a downward pressure on available supplies. This in turn leads to a further imbalance tilting towards water scarcity and the issues tied to that, including the degradation of water quality, and issues relating to prioritizing access (US EPA (c); Climate.gov). In many areas of the United States where water supplies are already thin and the water cycles and the ecosystems that they support are already delicate and in precarious states, climate change is seen as further putting those water syst ems and ecosystems at risk for systemic collapse (Frederick and Gleick), leading to potential conflicts (US Global Change Research Program). The problem relates to determining the impacts of climate change on the water supplies in the southern portion of the United States, detailing the impacts in the southeast and the southwest and undertaking a comparison and contrast and a general analysis of those impacts between the two halves. Related to this discussion are the kinds of interventions that are being undertaken, and how those interventions tie back to issues relating to sustainability of the interventions and the water supplies in the long run. That latter discussion with regard to the interventions, meanwhile, is done in the context of the academic literature on past interventions and how those interventions have fared, both within and outside of the United States. The problem itself of the impact of climate change on water supplies in the southern US is situated in the context of the water problem in the rest of the world, and the framework for sustainability that has been proposed to manage global and national water supplies (Postel and Richter; Pielou; Gerlak and Wilder; Bakker; US EPA). Turning to the problem contexts and background, climate change in general has been having a profound impact on the environment and on the key resources that sustain the populations of countries around the world.,The US is not an exception to this. The change in the ecosystems are breeding changes to the availability, quality, and access to vital resources, such as water. The effect is that where there are existing pressures on those resources in terms of availability and viability, those pressures are increasing. Moreover, the pressures are translating to new threats to the sustainability of the resource and of the human populations that depend on them. Situating the problem within a global context, one can see that since climate change impacts water quality and water availability for instance, different countries already on the brink of water crises are most vulnerable at present (Gerlak and Wilder; US EPA; US EPA (c)). Focusing on the United States,

Feminism in Iran Essay Example | Topics and Well Written Essays - 2000 words

Feminism in Iran - Essay Example Such a rejection in Islamic societies is often associated with the fact that it is seen as being part of western, middle class ideology and a very negative aspect of modernization. Modern day Iran is a strongly fundamentalist Islamic nation, at least as far as those who control it are concerned, with only 2% of the population being non-Islamic. Of the Muslims 89% are Sunnis and the rest are Shia. Despite all the elderly clerics who the media seem to concentrate upon Iran is a relatively young country with a median age of 28.6 years and only 5% of people living beyond retirement age.( World Fact Book, undated) Both sexes average the same length of schooling, but men out do women as far as literacy rates go – a difference of 13.1% in 2002. (World Fact Book) . The women have comparatively few children compared with many other countries – an average of 1.88 each. This is in part explained by an infant mortality rate running at almost 10 times that of the United Kingdom (World Fact Book), but also be the easy availability of contraception. What the figures do not show is how a society that was privately patriarchal has now become publicly patriarchal. Has women’s position really been changed as the post revolutionary state put in place a strongly patriarchal system of legislation – or is it just that the control over women has become more public – as the picture at the head of this piece seems to show? Less than 4 weeks after the downfall of the ruling family and the exit of the Shah, Khomeini was launching an attack on female Iranians. His timing was important - the eve of International Women’s Day - this was when he demanded a strict observance of Islamic dress codes.(Shahidian 2002, page 112) In June 2009 there were elections in Iran. The two main contenders were Mahmoud Ahmadinejad, the pro- polygamy incumbant, and Mir-Hossein Mousavi, a more liberal thinker who had vowed to remove laws which were oppressing women . However he did not win the election . In the period immediately followin g the Second World War many Islamic nations embraced modern , Western inspired ideas. ( Haddad and Esposito 1998, page ix) .These ideas included such things as educational patterns, legislation and social codes. Mnay women stopped wearing the veil and became increasing involved in public activities, and to a certain extent barriers between the sexes were

Wednesday, October 16, 2019

Water supply in the USA Research Paper Example | Topics and Well Written Essays - 2000 words

Water supply in the USA - Research Paper Example The observation is that climate change further worsens existing pressures on water supplies in all of the United States, relating to the way Americans routinely use water not just for consumption, but also for a host of economic activities, including agriculture, manufacturing, and the generation of power, among many uses. The general problem relates to the overall impact of climate change relating to elevating the demand for water in all areas of the United States, while at the same time exerting a downward pressure on available supplies. This in turn leads to a further imbalance tilting towards water scarcity and the issues tied to that, including the degradation of water quality, and issues relating to prioritizing access (US EPA (c); Climate.gov). In many areas of the United States where water supplies are already thin and the water cycles and the ecosystems that they support are already delicate and in precarious states, climate change is seen as further putting those water syst ems and ecosystems at risk for systemic collapse (Frederick and Gleick), leading to potential conflicts (US Global Change Research Program). The problem relates to determining the impacts of climate change on the water supplies in the southern portion of the United States, detailing the impacts in the southeast and the southwest and undertaking a comparison and contrast and a general analysis of those impacts between the two halves. Related to this discussion are the kinds of interventions that are being undertaken, and how those interventions tie back to issues relating to sustainability of the interventions and the water supplies in the long run. That latter discussion with regard to the interventions, meanwhile, is done in the context of the academic literature on past interventions and how those interventions have fared, both within and outside of the United States. The problem itself of the impact of climate change on water supplies in the southern US is situated in the context of the water problem in the rest of the world, and the framework for sustainability that has been proposed to manage global and national water supplies (Postel and Richter; Pielou; Gerlak and Wilder; Bakker; US EPA). Turning to the problem contexts and background, climate change in general has been having a profound impact on the environment and on the key resources that sustain the populations of countries around the world.,The US is not an exception to this. The change in the ecosystems are breeding changes to the availability, quality, and access to vital resources, such as water. The effect is that where there are existing pressures on those resources in terms of availability and viability, those pressures are increasing. Moreover, the pressures are translating to new threats to the sustainability of the resource and of the human populations that depend on them. Situating the problem within a global context, one can see that since climate change impacts water quality and water availability for instance, different countries already on the brink of water crises are most vulnerable at present (Gerlak and Wilder; US EPA; US EPA (c)). Focusing on the United States,

Tuesday, October 15, 2019

Comparison between two methods Essay Example | Topics and Well Written Essays - 3000 words

Comparison between two methods - Essay Example The most important rule of SSADM is that a system should be developed from the top down in hierarchical method and advanced to larger levels in feature. The design should initially reflect on the foremost activity of software, and then break this activity into sub activities and decompose each sub activities until the lowest level of detail has been achieved. The lowest level components illustrates the authentic giving out that will take place. In this manner all high-level logic and deign model are carried out earlier than detailed software code is written. If structured analysis has been carried out, the structured specification document can hand out as input to the design method (Kenneth, 1999). SSADM is now utilized as an expressive practice to additional argument of information systems development. SSADM is a group of conventional, methodical and certification principles for information systems development. SSADM comprises 5 core modules (Middleton, 2000): MERISE employs a data modeling procedure which recognizes the units concerned in the system, the association among these in addition to the practical reliability limitations associated to every relationship. The theoretical procedure model, examine a development as a position of process described from the position of observation of the administration policies of the corporation. This section will present the NIMSAD analysis and differentiate this technique from the MERISE methods for better system modeling and development. Now I will present the explanation of the SSADM methods through the Normative Information Model -based Systems Analysis and Design or NIMSAD model phases (Middleton, 2000). In this phase: it is stated that computers are â€Å"sensibly malleable† in the acumen that â€Å"they can be shaped and modeled to carry out several movement that can be distinguished in states of outputs, inputs and concerning logical procedures. This can direct to approach vacuums originated through

Shares and Joint Stock Companies in the New Economic Model Essay Example for Free

Shares and Joint Stock Companies in the New Economic Model Essay Introduction Good morning, dear colleagues. I’m glad to see everyone here. Thank you for your coming. Let me start by introducing myself. My name is Elena Torlopova. I’m a freshman of the State University of the Ministry of Finance of the Russian Federation. I study at the department of the international economic relations. My aim for today’s presentation is to give you information about Shares and Joint Stock Companies in the New Economic Model I plan to be brief. My presentation will last only 5 minutes. As you can see, my presentation is divided into 3 main parts. At first I would like to give you the basic concepts and characteristics of joint-stock companies. Then I would like to take a look at benefits and lacks of joint stock companies. Lastly we are going to discuss the definition of blue chips and consider a situation where they are used. Please interrupt me if there is something which needs clarifying, otherwise there will be time for your questions at the end of my talk. The main information about joint stock companies Joint-stock company (JSC) is a company, the authorised capital of which is divided into a certain number of shares owned by shareholders. Shareholders bear no responsibility for its obligations and run the risk, within the value of shares belonging to them, of losses associated with the companys activity. A joint-stock company in which shares can be  traded freely and which may have unlimited number of shareholders is an open joint-stock company. A joint-stock company which shareholders have a pre-emptive right to buy shares sold by other shareholders is a closed joint-stock company. Such a company has no right to hold a public subscription to its shares or for that matter offer them for sale to the general public. Advantages and disadvantages of joint stock companies The company provides so many advantages that it is widely popular all over the world. The main advantages are: Huge resources A company can raise large amount of resources from the genera public by issuing shares. Limited liability The liability of the shareholders is limited to the extent of the face value of the shares held by them or guarantee given by them. Diffused risk The entire business risk of a company is distributed over a large number of shareholders. Thus, the risk is reduced for each shareholder. Despite the above advantages, the company form of organisation also suffers from certain demerits. Oligarchic management It is controlled by a small group of Board of Directors who hardly protect the interest of other shareholders. Lack of secrecy It is very difficult to maintain business secrecy in a company because of every business strategy is discussed in the meeting of the Board of Directors and the annual accounts are published and compliance to Government. Fraudulent management The directors and managers may function for their personal gain overlooking the interest of the company. â€Å"Blue chips† Blue chip stock constitute shares or securities of large reliable companies with stable indices derived from income and dividends paid. In essence, the term blue chip is employed in the stock markets by analogy to describe the actions of well-established entities. The term was coined by Oliver Gingold of Dow Jones Company in 1923. It is reported that  the term was born when Gingold was taking note of several businesses with shares priced at $ 200 per share or more. And went on to indicate intentions of writing an article about the blue chips. Thus the term was born. Typically, blue chips are indicators of the overall market. Blue chip shares are the most liquid securities market. The list of blue chip stock changes periodically, but there are always those that manage to stay on top for years. Examples of the western blue-chip companies are Apple, IBM, The Coca-Cola Company, Ford, Google, etc. In Russia blue chips occur mainly in the oil, gas, energy and telecommunications companies. In particular, the blue chips include such companies as Gazprom, Lukoil, Norilsk Nickel, Rosneft, Sberbank, Rostelecom, RusHydro, Polyus Gold, etc. These companies typically have leading positions in the RTS and MICEX. As you can see on the pie chart 15 percent of blue chips belong to Gazprom. It means that Gazprom shares are the most liquid in terms of sales and account for 15 percent of the total trading volume on the market. The second largest sales shares owned by Lukoil. They constitute 14, 02% of total trading in the market. Sberbank is the third after a Gazprom and Lukoil amounts to 13.88% of sales of its shares on the market. Norilsk Nickel and Rosneft makes 7.56% and 7% of sales in the market, respectively. And finally Surgutneftegas and VTB Bank constitute 4,82% and 4,28% of sales in the market. Thus the total weight of seven securities in the index is 67%, which implies that it is these securities provide direction and determine the dynamics of the Russian market as a whole. Conclusions We gave the definition of the concept of joint-stock company and it basic characteristics. Then we looked at the advantages and disadvantages of joint stock companies. And in the end we discussed the stock market, and specifically the blue chips and looked at the dynamics of the sales of seven major blue chips Russia. So, in conclusion I would like to say that joint-stock form of entrepreneurship plays an important role in the formation of normal conditions of operation of enterprises, allows shareholders to raise funds for its activities, and each worker can become the owner of the company, through acquisition of shares, each shareholder reduces the risk of losing a significant amount of money (each at risk only to the extent of its sum shares) allows you to work more efficiently, since  all are interested in making profits and dividends It should be emphasize once again that the joint stock company is a unique form of collective ownership of the implementation, where everybody i nterested in the results of its operations. Bibliography * http://en.wikipedia.org/wiki/Joint-stock_company * http://en.wikipedia.org/wiki/Blue_chip_(stock_market) * http://www.bukisa.com/articles/444625_basic-overview-of-blue-chip-stock

Monday, October 14, 2019

Effects Of Improper Waste Management Environmental Sciences Essay

Effects Of Improper Waste Management Environmental Sciences Essay The issue of Waste Management has be an ongoing issue in Nigeria but came into lime light in 1988 with the disposal of toxic waste in the then Delta State (Edo state). Nigeria as the most populous country in Africa has an estimated population size of about 160 million people, a growth rate of 2.37% and generates an annual municipal waste figure of 25,000,000 tones. This policy sets to identify the probable sources of waste generation, their effects, and disposal methodology and thereafter recommend mitigation strategy for Waste Management by proffering probable solution to its management by means of: Its Prevention, Its Reduction, Its Beneficial usage through the 3Rs of waste management and Development of Statutory, legal and legislative regulations for the management of waste. Although there are Federal and State Ministry of Environment, Environmental Protection Agency (State and Federal), we still have problems about enforcement of the responsibilities for regulation and monitoring to ensure legal, statutory and social compliance. COUNTRY PROFILE The Nigerian economy is one that has experienced a number of environmental problems in its over fifty (50) years of being an independent nation. There apparently seem to be no end in sight except a few radical changes are made to its economical and environmental policies. The waste management system in operation in Nigeria leaves a lot to be desired. The countrys current environmental issues include but are not limited to: Oil pollution and gas flaring (which affect water, aquatic lives, air, and lead to loss of arable land), Waste pollution (which causes air, land and water pollution), Rapid urbanization. A look at nine (9) of the major cities in Nigeria gives a fairly good idea of the disaster the country is looking at in terms of unchecked waste generation and management. The Map below shows each of the nine (9) states with their generated annual municipal waste One would expect that a country of such magnitude both in population size and economic importance will have structures in place to adequately combat the menace posed by waste generation and management but this is not the case as the country doesnt have a single sanitary landfill for waste disposal. It disposes waste in open landfills and dumps. SOURCES OF WASTE GENERATION The environment is a continuously changing one and the quantity of waste generated increases with an increase in population, peoples attitudes and rapid urbanization. In providing a solution and putting in place a policy to manage waste in the Nigerian economy a clear understanding of what waste is and how it is generated is required. What is waste? The United Nations Statistics Division (UNSD) defines waste as materials that are not prime products (that is products produced for the market) for which the generator has no further use in terms of his/her own purposes of production, transformation or consumption, and of which he/she wants to dispose. Waste is generated from: Residential waste: household waste and kitchen waste Commercial waste: office waste e.g. papers Institutional waste: papers Municipal waste: sludge and general waste Process waste: scraps Agricultural waste: pesticides Construction waste: wood, Concrete etc Drilling: Oil spillage, gas flaring, coal, ash Types of waste (Diagram) EFFECTS OF IMPROPER WASTE MANAGEMENT It is a well known fact that our environment is poorly protected and not many Nigerians know the actual scope and the ramifications of this problem. What is Waste Management? Waste management is the act of collection, storage and disposal of waste to prevent pollution, degradation of the environment. This ranges from ordinary household, industrial wastes even generated by factories and nuclear power plants. Proliferation of vermin: Dirt has been known to be the breeding grounds for vermin like rodents, mosquitoes and microbes Water pollution: This occurs mainly when ground water is contaminated from oil spillage and washed off chemicals that enter the ground. This has an adverse effect on marine life, drinking water and deforestation occurs because plants get contaminated water into their roots. Air pollution: This occurs when waste is incinerated indiscriminately in public without the proper facilities being used and even dumped in the open. The incinerated waste especially plastic release toxins that could produce acid rain and the smoke could cause lung damage. Land pollution: This occurs when waste is not deposited at proper disposal sites but is dumped in open landfills. This causes a blot on the landscape and a reduction in property price where such landfills are found. Public Health Issues: The unregulated dumping of waste which gives room for microbes and insect to breed creates health issues such as: malaria (mosquitoes), typhoid (contaminated home water source) etc. Reduction in Agricultural production: waste especially oil spillage, gas flaring, chemical contaminants reduce the quality of the soil and this in turn is noticed in the reduction of agricultural produce Methane production: which if not treated properly causes green house effect and add to ozone depletion (unmanaged waste smells and generates the gas) Climate change leads to an increase in the intensity and frequency of weather extremes, such as heat waves, floods, droughts and tropical cyclones. The people hardest hit by climate change and environmental degradation are those living in the most vulnerable areas such as coastal communities and Delta Regions in Nigeria. This climate change and the effects it generates lead to property damage, business interruption, forest fires and health risks. Flooding: This occurs when waste is disposed in drainages by man or by natural occurrence (washed by rain).This could causes flooding when rain falls. Erosion: The presence of chemical and toxic waste in the soil reduces its compatibility and causes soil wash off when rain falls. PROBLEMS: The problem of inadequate waste disposal and management practice in Nigeria is based on a few controllable factors that are highlighted as: Enlightenment: Nigerias citizens lack the general awareness of worlds best practices for waste disposal, management and the possible effects of non-compliance on their health. Meager funding: The funds allocated to each state for waste management is inadequate to battle the issues of environmental waste. States like Lagos have only 5-7% of the states allocation to battle waste management. Obsolete technology and inadequate equipment: The people who have been given the job of waste disposal and management do not have the equipments and technology to do the job. Inexperience of staff: The staff who have been allocated to manage waste disposal and management do not have the technical knowhow to go about doing the job. Incomplete policy to combat improper waste disposal: The policies on ground have not been improved to meet the changing population size, urbanization and worlds best practice. Sanitary Landfills: Nigeria as a country doesnt have a single sanitary landfill to its name. It uses open landfills and dumpsites for its waste disposal which is not hygienic and environmentally friendly. Traffic: This situation is more prominent in urban cities where the distance between the point of collection of waste and the disposal point could take a whole day to cover because of traffic congestion Legislature implementation: Nigeria has the uniqueness of making laws without having the legal presence to implement them and has cascaded to the waste management sector. Government Legislation: In situations where there are environmental laws in place, the fines attributed to breaking the laws are not a fair representation of the offence committed.eg Environmental Sanitation Edict NO:12.1985 stipulated a fine of two (200 ) naira or two( 2) months in prison for going out on sanitation days. GOVERNMENTS EFFORT SO FAR It is accepted that the Nigerian government has put in place several environmental bodies to curb the increasing environmental issues caused by waste generation. Such bodies include: Federal Environmental Protection Agency (FEPA) was established on 30th December, 1988 by the Federal Government to secure a quality environment for Nigerians. National Environmental Standards and Regulation Enforcement Agency (NESREA) was established in 1999 to enforce environmental laws. It was established as a subset of FEPA. Each state in Nigeria has its own Environmental Protection Agency e.g. Lagos State Environmental Protection Agency (LASEPA) State Ministry of Environment National Oil Spill Detection and Response Agency (NOSDRA) ADVISED SOLUTIONS (RECOMMENDATIONS) GOVERNMENT TECHNICAL SOLUTION. The technical solutions recommended for government approval are: Prevention of waste This technique will include practices like: increasing the shelf life of a product (increasing product warranty) by citizens in their everyday life. Waste Generation Reduction. The key to achieving waste generation reduction is for the government to create awareness. Educate people on the effects of waste generation on the economy, the environment and themselves and to advise them on waste generation reduction techniques of shopping. This technique says that: People should only purchase what they need (necessities and essentials) People should purchase reusable products and goods People should buy products with little packaging People should buy recyclable products Composting: The government should set up waste compositing sites at strategic parts of the country. Compositing involves the use of anaerobic bacteria at high temperature and in the presence of Oxygen to digest and breakdown biodegradable waste into different components that are harmless to the environment and the end product can be used as fertilizers by farmers. Recycling The Nigerian government has no recycling department or unit in its environmental agency and also has no recycling plant. The key to recycling is in educating people on the different classes of waste and the different means of disposal and separation. The process of recycling involves the reprocessing of waste to produce a different or new product. This process should involved both the government and the people Incineration: With the reduction of the water content in generated waste, incineration can be carried out in an enclosed space under certain conditions to generate heat and energy that can be used to generate electricity. The government should look at this option as another means for energy generation as with the population size in the country the waste generated will be put to better use. This can only be achieved if citizens develop the practice of classifying waste into: general waste, recyclable waste and reusable waste. Sanitary landfill: Nigeria as a country doesnt have a single sanitary landfill. A sanitary landfill is usually used for the final waste that cannot be reused, recycled or composited. The ground is dug and lined to prevent the contamination of ground water and the waste is packed and lined then covered with dirt to reduce its smell and to stop animals from digging it up. The sanitary landfill site should be located far from residential areas. The government should look at building a few sanitary landfills in key areas in the country GOVERNMENT GENERAL SOLUTION: Set up awareness campaign to educate people on the worlds waste disposal and management best practices Provide technical knowhow and training programmers to environmental waste management agency employees Companies and the type of waste they generate should be registered with the government so as to enable the government put facilities on ground to dispose and manage the waste. Different types of waste should have different disposable sites. The disposal and management responsibility of waste should be cascaded down. The government should get private investors who will assist them in managing the generated waste Encourage the generation of a professional body for waste disposal and management Ensure that there is accountability for any waste disposal issue that might arise in the country. Ensure waste policys are implemented Ensure that physical, informative and financial facilities are put on ground to adequately combat environmental waste disposal and management. Creation of an adequate legal frame work to try offenders of waste management laws will go a long way in. Finance research into waste reduction and waste utilization technique ECONOMIC BENEFITS OF RECOMMENDATIONS Methane: The methane generated from fermentation if managed properly can be used as cooking fuel. This the government can add as another source of cooking fuel instead of kerosene and firewood Re cycling: Stops flooding: Incineration: Heat and Energy generated through this process will be used in electricity generation which in turn reduces our reliance on hydrolic electricity generation Compositing: This is a source of fertilizer to farmers that is environmental friendly Agriculture: The improvement in soil quality will lead to a corresponding increase in agricultural produce which if exported is a source of foreign currency. Reduces erosion: The increase in comportment of the soil will reduces the probability of erosion occurring with rainfall Good ground water and aquatic life survival: This will provide clean water for human consumption and plant growth Healthy populace: With the advent of a sustainable environment the countrys population will be healthy and free of waste and water borne diseases.

Sunday, October 13, 2019

sport marketing :: essays research papers

Sport Marketing The notion of "marketing myopia" has haunted marketers since Theodore Levitt published his famous article "Marketing Myopia" in Harvard Business Review in 1960. Levitt argues that companies which narrowly focus on the product to the detriment of customer requirements (i.e., dispensing with the marketing concept) suffer from marketing myopia. Myopia or shortsightedness is often apparent within organizations. Several types of marketing myopia can be identified including classic myopia, competitive myopia and efficiency myopia. Companies displaying one of these three elements are clearly distinguishable from innovative firms which embrace the marketing concept in practice and which have a much broader scope than is required for a single business sector. In order to overcome myopia and become innovative, the following is recommended:  Companies should hold a generic view of themselves and of the industry in which they operate  Companies should also take the crucial step to monitor other industries  Benchmarking should be conducted  Marketing staff should be recruited but not necessarily from the same sector  Companies should be flexible and seek unique solutions. The biggest reason for market myopia is the lack of research or the inefficiency of that research. Almost all of the myopia could be avoided if research and the researchers were trained and the thought process was developed in a good fashion. All of marketing whether it is in sports or out of sports all revolves around the same basic principles the four P’s. In all of marketing the P’s are: Product, Price, Place and Promotion, however in sport there is an added principle and that is Public relations. Even the best marketers can come up short according to these principles because it does not matter how much you know but rather in a service business such as sport it most important to be able to interact with the consumers. A great example of a lack of interaction is here at Merrimack College. Our sport programs do not get enough marketing coverage both on and off campus. Our Sports Information Director does not place flyers in the student union and also he does not advertise on the college television channel. Here at Merrimack, they lack the Promotion aspect of their sports, which includes our division one hockey program. A great example of this lack of communication was the fact that when the hockey team was playing against then #1 Maine, the campus was not informed of this huge game.

Friday, October 11, 2019

The Horrors of Animal Euthanasia Essay -- Euthanasia Physician Assiste

The Horrors of Animal Euthanasia   Ã‚  Ã‚   Due to the domestication of cats and dogs their populations have skyrocketed. This is due in part to the lack of pet owners acting in a  responsible manner. These responsibilities include the spaying and neutering of   pets. These numbers of homeless animals in communities have caused humane societies to euthanize too many animals. This, I feel is a violation to animal rights and is a cruel way for these animals to have to leave this world. I disagree with the activists who believe that the way to keep animal populations down is to euthanize these poor animals. Nothing good could come from the slaughtering of these animals. Fostering large numbers of animals in communities is an overwhelming task, but there are many solutions to this problem. The euthanizing of animals is wrong and all shelters should switch to a no-kill policy.   Ã‚  Ã‚   There are too many animals in the shelters. Animal control officers are forced to put these animals to sleep that could have made loving and affectionate pets. ( Open Your Hearts Mission, p.1 ). Last year at the Vanderburgh Humane Society 2022 unwanted cats and dogs were put to death. These animals were taken to the E-room in the back where   they were injected with a syringe of cool-blue liquid death. After a few seconds the animal fell limp and eventually its heart stopped. Employees at the humane society have the task of deciding which of these animals should be euthanized. This is not something they enjoy and take pride in doing. Usually after a few days the animals which have not been adopted will be euthanized. The ones that are sick or hurt are the first ones to go. The events that take place in the process of the euthanization of... ... http://www.npsc.nbs.gov/resource/othrdata/crp/crp.htm Executive Summary.   (1997).   Fish and Wildlife Service   Internet. http://www.fws.gov/~r9mbmo/issues/arcgoose/parti/execsum.html The Goose Crisis.   (1997).   The Goose Crisis   Internet. http:www.und.nodak.edu/org/ndwild/sgcrisis2.html Kantrud, Harold A., Rolf R. Koford, Douglas H. Johnson, and Michael D. Schwartz. (1993).   The Conservation Reserve Program  Ã‚   Internet.   http://www.npsc.nbs.gov/resource/othrdata/crp/crp.htm Mountain Lion.   (1997).   Kidsfarm   Internet.   http://www.kidsfarm.com/lions.htm Too Much of a Good Thing.   (1997).   Internet.   http://udwild.psych.und.nodak.edu/HTMLPages/goosecrisis.html Trophy Hunting of Mountain Lions: A History of Deception.   (1995).   California  Ã‚   Mountain Lion   Internet. http://www.sierraclulb.org/chapters/ca/mountain-lion/history.html

Lung Cancer Detection Methods

There are several ways in which lung cancer may be detected and some of these are the following: First of all is to have the patient undergo what is technically referred to as â€Å"screening† (The Health Alliance, 2006, n.p.). Here, a series of laboratory tests and other examinations are carried out (The Health Alliance, 2006, n.p.). Second is known as blood test (The Health Alliance, 2006, n.p.). Here, the blood is placed under scrutiny to see if the â€Å"lung cancer† has multiplied and have already reached the patient’s liver or bones (The Health Alliance, 2006, n.p.). Third is the â€Å"bone marrow biopsy† wherein a needle is utilized to take out a small portion of the bone located at the â€Å"back of the hip bone† and is placed under the â€Å"microscope† to see if any cancer cells may be detected (The Health Alliance, 2006, n.p.). Fourth is technically referred to as â€Å"mediastinoscopy† (Harvard University, 2008, n.p.). Here, the neck is incised to allow a lighted pipe to go through and to eventually get a sample of tissue inside it (Harvard University, 2008, n.p.) Again, this will confirm if cancer cells are there or not by looking at it under the microscope (Harvard University, 2008, n.p). Fifth is known as â€Å"bronchoscopy† (The Health Alliance, 2006, n.p.). Like the aforementioned fourth detection method, a lighted pipe is involved here as well (The Health Alliance, 2006, n.p.). However, with this method, the tube will be inserted on the nose and will look for tumors or obstructions in the lungs (The Health Alliance, 2006, n.p.). It may also take tissue samples or fluids so that it may be checked under the microscope for cancer cells’ presence (The Health Alliance, 2006, n.p.). Last but not least is technically referred to as â€Å"needle biopsy† (Radiological Society of North America Inc., 2008, n.p.). Here, the lungs are monitored through a machine known as â€Å"CT Scan† while a needle is utilized to take out a mass sample in the lungs (Radiological Society of North America Inc., 2008, n.p.).Like in the other methods, the aforementioned sample will have to be checked under the microscope (Radiological Society of North America Inc., 2008, n.p.). References Harvard University. (2008). Mediastinoscopy. Retrieved February 14, 2008 from http://www.health.harvard.edu/diagnostic-tests/mediastinoscopy.htm Radiological Society of North America, Inc. (2008). Needle Biopsy of Lung (Chest) Nodules. Retrieved February 14, 2008 from http://www.radiologyinfo.org/en/info.cfm?pg=nlungbiop&bhcp=1 The Health Alliance. (2006). Lung Cancer Detection. Retrieved February 14, 2008 from http://www.health-alliance.com/Cancer/lung/detection.html   

Thursday, October 10, 2019

Dna Analysis Practical Write-Up

Title: DNA analysis Aim: a) Isolate and Purify Bacterial Chromosomal DNA from a strain of E. coli b) Visualization of restriction fragments by Agarose Gel electrophoresis Objectives: * to isolate and purify bacterial chromosomal DNA from a strain of E. coli * to analyze and identify DNA by use of a spectro-photometer * to use restriction enzymes to cleave DNA into fragments * to visualize the restriction fragments by gel electrophoresis * to compare the different DNA fragments generated by use of molecular markersAbstract This work describes a lysis method for the isolation and purification of bacterial genomic DNA and visualization of the restriction fragments by agarose gel electrophoresis. It was noted that for one to isolate and purify bacterial chromosomal DNA several steps are taken into consideration. DNA was found to absorb at 260nm wavelength in a UV spectrophotometer. Restriction enzymes were added to cleave DNA which would produce various DNA fragments. DNA can be separate d into different sized fragments by gel electrophoresis.The bacterial DNA was successfully isolated and purified however it could not be observed after running the gel. DNA analysis is a standard practice for defining paternity or maternity, predisposition to disease, embryonic health and criminal guilty. But in our context, DNA analysis is mainly used for predisposition of diseases in bacteria. Bacteria are pathogenic microorganisms that cause infectious diseases including cholera, syphilis, anthrax and leprosy. The most common fatal bacterial diseases are respiratory infections such as tuberculosis (Barnum S.R; 1998). Nucleic acids encode information relating to cell structure and function. Cells have the ability to make copies of their DNA and pass this information to daughter cells. Nucleic acids are polymers of nucleotides. Nucleotides are composed of ribose (a 5` carbon) sugar and either a purine and pyrimidine base at 1` position. The purine bases are adenine (A) and guanine (G) and the pyrimidine bases are cytosine (C), thymine (T) and Uracil (U). Uracil is only found in RNA and thymine is only found in DNA (Wiser M. F; 2002).Isolation of nucleic acid – three major types of techniques are employed in the isolation of nucleic acids differential solubility, absorption methods or density gradient centrifugation. The choice of method will depend on the type of DNA being isolated and the application. A major goal of nucleic acid isolation is the removal of proteins. The separation of nucleic acids from proteins is generally accomplished due to their different chemical properties. In particular, the highly charged phosphate backbone makes the nucleic acids rather hydrophilic as compared to proteins which are more hydrophobic (Allison L.A; 2012). Spectrophotometry is a versatile analytical tool. The underlying principle of spectrophotometry is to shine light on a sample and to analyze how the sample affects the light. DNA absorbs light at a wavelength of approximately 260nm (Stryer; 2006). Centrifugation is a process that involves the use of the centrifugal force for the separation of mixtures. Separation is based size, shape and density. It utilizes density difference between the particles/macromolecules and the medium in which these are dispersed (Gupta P. K; 2006).Dispersed systems are subjected to artificially induced gravitational fields. A buffer is an aqueous solution consisting of a mixture of weak acid and its conjugate base or weak base and its conjugate acid. Its pH changes very little when a small amount of strong acid or base is added to it and thus it is used to prevent any change in the pH of a solution (Cowan M. K; 2009). Electrophoresis is a diverse technique of separation used to separate and sometimes purify macromolecules especially proteins and nucleic acids that differ in size, charge or conformation by an electric current (Stryer L. 2006). Gel electrophoresis refers to using a gel as an ant convective mediu m and or sieving medium during electrophoresis. Gel electrophoresis is most commonly used for separation of biological macromolecules such as deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or protein; however, gel electrophoresis can be used for separation of nanoparticles. Materials Used * Luria Broth medium * SET Buffer * TEN Buffer * Choloroform/isoamyl alcohol. 24:1 mixture * Phenol/ chloroform 1:1 (Buffer saturated phenol) * Ethanol (95%) stored at -20? * Na Acetate * NaCl: 5M sterilized by autoclaving Sodium dodecylsulphate (SDS) : 26% (w/v) * Bacteria cells * Plastic test tubes * Glass rods * Wide bore pipette * Ice bath * Centrifuge * Ethidium bromide * Agarose * TBE buffer Methodology Each group carried out the following procedures: Used two 50ml sterile plastic tubes, harvested cells by centrifugation for 10 min 4’C. Combined pellets to give approximately 1g wet weight of cells. Washed the pellet, re-suspended it in 20ml Ten buffer by gentle vortexing. Harvest ed the cells again as described above. Re suspended the cells in 10ml of Set buffer and let them sit on ice for 5min.Added 1000Â µL of lysozyme and incubated at 37? for 30 min. Divided the cell suspension into two in separate sterile 50ml tubes. Added 5 ml Ten Buffer and 500Â µl of SDS. Gently mixed the tubes by inverting them until lysis occurred. To each tube added 1ml 5M NaCl and an equal volume of buffer saturated phenol. The tubes were inverted till the mixture was emulsified. Separated the phases by centrifugation for 10min at 40C. Recovered the upper aqueous phase using a wide bore pipette. When retaining the aqueous phase the pellicle at the interface was avoided. Repeated the extraction until the interface was clear.Added an equal volume of chloroform and extract residual protein as described above. Transferred the upper aqueous phases from both tubes to a 100ml beaker. Set them on ice and added 1/10th volume 3M Na acetate. Precipitate the DNA by addition of 2 volumes of ice cold 95% ethanol. Mixed thoroughly and allow it to stand for about 5min on ice for the DNA to precipitate. Spooled the DNA out of solution on a glass rod, dipped it into a tube of 95% ethanol and re-suspended in 10ml Ten Buffer. Left to dissolve overnight at 4’C B) Gel electrophoresis The gel was prepared by melting 1. 6g of agarose plus 200ml of 0. x TBE buffer. Swirled the mixture and allowed it to cool to 55?. Added 10? l ethiduim dye Loaded the gel in the following order; 1. Undigested pBSK 2. pBSK + digested with Eco R1 and Xba 1 3. Undigested DNA from a blue colony 4. DNA from a blue colony digested with Eco R1 and Xba 1 5. Undigested DNA from a white colony 6. DNA from a white colony digested with Eco R1 and Xba1 7. Lambda Hind III molecular weight markers After loading the gel it was run at 100 volts for 2 hours. Results We managed to precipitate DNA out of the Bacterial cells. DNA was seen a small white like fragments.However we could not spool the DNA out of sol ution using glass rods due to fact that DNA is a fragile compound hence when we twisted / spooled for DNA we destroyed the DNA strands cutting them into smaller fragments. The following day, analysis of the DNA sample in a spectrophotometer was carried out. It was found that DNA absorbed a specific wavelength of 260nm. This proved the presence of DNA in the sample. Our sample was digested by restriction enzymes and labeled the DNA fragments with an identification dye and ran them on the Gel electrophoresis together with molecular weight markers.After running the gel no observeable bands of different band fragments were observed. Only the molecular weight markers bands were observed. Discussion The TEN and SET buffer were used to lyse the cells. They are good buffering agent, which solubilizes the DNA, while protecting it from degradation. Eluting and storing the DNA in TBE Buffer is helpful if the EDTA does not affect the downstream applications. EDTA chelates or binds to Mg2+ ions present in purified DNA and can help inhibit possible contaminating nuclease activity (Cowan M. K; 2009).Balancing of test tubes before centrifugation in order for the centrifugation process to be effective to create centrifugal field that results in maximum separation of cell components. According to Wiser M. F 2002, DNA is very insoluble in ethanol and isopropanol, but both alcohols are very water soluble. Thus, it will dissolve in water to form a solution and cause the DNA in the solution to aggregate and precipitate out. Isopropanol is often better to use because it has greater potency in precipitating the DNA and thus lower concentration is required. This is advantageous because it will take less time for the isopropyl alcohol to evaporate.Salts such as sodium chloride and ammonium acetate remove histone and non-histone chromosomal proteins bound to the DNA. As soon as 95% ethanol was added after sodium acetate for DNA precipitation, the whole solution turned cloudy with a lot of white precipitate, precipitating down. According to Allison L. A, 2012; sodium acetate which is negatively charged and low pH was used which contributes to charging positively the DNA. A combination of this plus high salt molarity enhances formation of aggregates of DNA and facilitates the pelleting procedure. Chloroform isoamyl-alcohol is a type of detergent.It binds to protein and lipids of cell membrane and dissolves them. By this it disrupted the bonds that hold the cell membrane together and cause it to breakdown. It then forms complexes with these lipids and proteins, causing them to precipitate out of solution (Besty T and Keogh J; 2005). This reduced chance of contaminated DNA being obtained hence making it possible for us to be able to precipitate DNA only. Alcohol (95%ethanol) is used to precipitate DNA. SDS which stands for ‘sodium dodecyl sulfate' is a strong anionic detergent that can solubilize the proteins and lipids that form the membranes.This will helped t he cell membranes and nuclear envelopes to break down and expose the chromosomes that contain the DNA. In addition to removing the membrane barriers, SDS helped release the DNA from histones and other DNA binding proteins by denaturing them (Barnum S. R; 1998). Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. When exposed to ultraviolet light, it will fluoresce with an orange colour, intensifying almost 20-fold after binding to DNA (Wiser M.F; 2012). Molecular weight size is a set of standards that are used to identify the approximate size of a molecule run on a gel. These markers were composed of nucleic acids of different sizes. A few reasons you may not see bands on the gel after electrophoresis: When preparing the gel for electrophoresis TBE buffer was used. This was done so that the temperature can be maintained and lubricate the electrolyte. L oading dye was added this helped weigh down the DNA so that it can sink into the bottom wells and not float in the buffer solution. According to Gupta P.K, 2006; loading dye moves quickly than the actual DNA parts so it is an indicator to when to turn off the power on the electrophoresis chamber. The dye also makes the DNA visible to the naked eye, giving it a purplish color and making it easier to work with. After Gel electrophoresis no bands of DNA were observed. This according Allison L. A (2012) might have been as a result of any of the following * DNA concentration might have been too low. * DNA sample is contaminated with RNA and Protein * DNA bands are too small and have run out of the gel The buffer system in which the gel is suspended is not doing its job correctly. The buffer might have to be made fresh. * The electrophoresis apparatus is not in the correct orientation (electrodes not connected to the right poles). The major drawback in the experiment was that our fellow c olleagues were not able to isolate and purify their DNA. Also when working with DNA temperature regulations were not sometimes adhered to, it was sometimes left on the surface tables for long periods esp. when the samples were being analyzed in the spectrophotometer.Recommendations With proper teamwork and co-ordination among my fellow classmates much larger quantities of DNA could have been isolated and purified. The DNA should not be kept at room conditions for a long time. Conclusion The experiment was partly a success managed to isolate and purify DNA, analyzed it using a spectrophotometer. However bands of DNA could not be visualized after running the gel. References 1. Allison L. A. (2012). Fundamental Molecular Biology, 2nd edition. Denvers. John Wiley and Sons Inc. 2. Barnum Susan.R, (1998), Biotechnology: An introduction, New Delhi, Vikas Publishing House. 3. Besty Tom and Keogh Jim, (2005), Microbiology demystified, New York; MacGraw-Hill. 4. Cowan Majorie Kelly, (2009), M icrobiology: A Systems Approach, 3rd edition; New York; MacGraw-Hill. 5. Gupta, P. K. (2006). Elements of Biotechnology, Meerut. Rastogi Publications. 6. Stryer L, Berg J. M and John Tymozcko. (2006). Biochemistry. 5th edition. California. W. H Freeman and Company. 7. Wiser, M. F. (2002). Methods in cell biology. Berlin. Springer Verlog CHINHOYI UNIVERSITY OF TECHNOLOGYName Tanyaradzwa R Ngara Reg Number C1110934J Course Recombinant DNA Technology Module Code CUBT 203 Program Biotechnology Level 2:1 Lecturer Dr Mlambo Practical Write-up DNA analysis

Wednesday, October 9, 2019

Health and Safety Assignment Example | Topics and Well Written Essays - 500 words

Health and Safety - Assignment Example First, the employees stop feeling proud to be associated with the company. Secondly, the careless accidents can instill fear on the employees on learning how they are working in poor conditions. Additionally, high cost-cutting and reduced infrastructure budget exposes the employees to more workplace hazards. Finally, these practices make the employees to lack role models. First, they can lead to fatal accidents that cause massive ecological damages, like the Gulf of Mexico oil spill in 2010, which can lead employees to lack confidence in the company (Balaguer, 2010). Secondly, they can lead the employees to take court action against the company. Thirdly, the employees may plan mass protest or strike which can paralyze the operations of the company. In addition, they can lead accidents, which cause death and injury to employees straining the relationship between the two parties. Lastly, they can lead the employees to question the company commitment to their safety and wellbeing. First, the BP operations license may suspend. Secondly, the company license may be revoked due to failure to meet the standards set. Thirdly, the authorities can close part of BP facilities they deem hazardous, like it happened in 2006 on some part of Prudhoe Bay oilfield in Alaska (Coy and Reed, 2010, p.53). Additionally, huge fines can be imposed on the company. Finally, the authorities make sure that the company cleans up the mess from accidents, pays damages, and contributes towards restoration of the ecology. First, they cost the company’s reputation a major blow once revealed. Secondly, the customers loose confidence in the company’s products turning to competitors. Thirdly, the public and customers may sue the company due to environmental damages. Further, the public and customers may shun the company’s social responsibility efforts. Lastly, due to